During development, discrete organs and entire body plans emerge from the coordinate actions of individual cells. These complex morphogenetic events require dynamic regulation of cell shape, polarity and adhesion across cell populations. Our lab seeks to understand how these diverse cellular behaviors are orchestrated to produce an organ's functional shape.
To this end, we are using genetic, cell biological and live imaging approaches in Drosophila to investigate how a simple, ovarian structure called and egg chamber lengthens as it grows. We are particularly interested in the roles that collective cell migration and basement membrane remodeling play in this process. Please visit our lab website for more information about the strengths of this system and the specific questions that we pursue.
Barlan, K, Cetera, M, and Horne-Badovinac, S. (2017). Fat2 and Lar define a basally localized planar signaling system controlling collective cell migration. Developmental Cell, 40, 467-477. (PubMed)
Isabella, AJ and Horne-Badovinac, S. (2016). Rab10-mediated secretion synergizes with tissue movement to build a polarized basement membrane architecture for organ morphogenesis. Developmental Cell, 38 (1), 47-60. (PubMed)
Andersen, D and Horne-Badovinac, S. (2016). Influence of ovarian muscle contraction and oocyte growth on egg chamber elongation in Drosophila. Development, 143, 1375-1387. (PubMed)
Isabella, AJ and Horne-Badovinac, S. (2015). Dynamic regulation of basement membrane protein levels promotes egg chamber elongation in Drosophila. Developmental Biology, 406, 212-221. (PubMed)
Cetera, M and Horne-Badovinac, S. (2015). Round and round gets you somewhere: collective cell migration and planar polarity in elongating Drosophila egg chambers. Current Opinion in Genetics & Development, 32, 10-15. (PubMed)
Cetera, M., Ramirez-San Juan, G.R., Oakes, P.W., Lewellyn, L., Fairchild, M.J., Tanentazapf, G., Gardel, M.L. and Horne-Badovinac, S. (2014). Epithelial rotation promotes the global alignment of contractile actin bundles during Drosophila egg chamber elongation. Nature Communications, 5:5511. (PubMed)
Horne-Badovinac, S. (2014). The Drosophila egg chamber – a new spin on how tissues elongate. Integrative and Comparative Biology, 54(4), 667-676. (PubMed)
Lewellyn, L., Cetera, M. and Horne-Badovinac, S. (2013). Misshapen decreases integrin levels to promote epithelial motility and planar polarity in Drosophila. Journal of Cell Biology, 200(6) 721-729. (PubMed)
Lerner, D.W., McCoy, D., Isabella, A.J., Mahowald, A.P., Gerlach, G.F., Chaudhry, T.A. and Horne-Badovinac, S. (2013). A Rab10-dependent mechanism for polarized basement membrane secretion during organ morphogenesis. Developmental Cell 24, 159-168. (PubMed)